Recombinant Human Epidermal Growth Factor(rhEGF)- R&S Pharmchem Co., Ltd

Recombinant Human Epidermal Growth Factor(rhEGF)

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Name: Recombinant Human Epidermal Growth Factor (rhEGF)

Introduction:
rhEGF is a soluble non carbohydrate protein, which contains 53 amino acids. The composition, sequence and peptide map of rhEGF are strictly accord with the natural hEGF. The Amino acid sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been completely removed enzymatically.

Molecular weight: 6216Da
Characteristics: White powder, PH 6.5-7.5, Isoelectric point 4.6, acid tolerance(PH=1.0), stable when heated.

Bioactivity: 1.00X10⁶ IU/mg protein Min
Purity: 95% Min and 98%(HPLC and SDS-PAGE analysis)Min

Expression and purification system:
With the application of a high-effective secretive expression system of Escherichia coli, target protein was secreted directly into the culture medium after membrane modification. Extremely high bioactivity achieved due to the remaining of advanced protein structures. By using full sets of premium separation and purification system, purity of the production reached above 95%, and the highest can be above 98%.

Application:
rhEGF can promote the treatments of the wounds of skin and cornea, and has the potential of repairing wounds in alimentary tract, kidney, liver, lung and trachea. It can be used in the treatments of burns, all kings of surgical and postoperative wounds and the injury or ulcer of cornea. Recommended concentration - 0.1-1.0 ng/ml medium depending on cell type

rhEGF can enhance the growth of new epidermal and stem cells, promote the cell's metabolism and converse differentiation. It is widely used in cosmetics such as whitening, anti-crinkle, crinkle dispelling, anti-aging, etc.

Biological Activity:
ProSpec's recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is less then 0.1 ng/ml corresponding to a specific activity of 1 x 1000000 Units/mg.

Storage:
2-8℃(in refrigerator) storage, stable within 36 months.

Endotoxin:
Less than 0.1 ng/μg (IEU/μg) of recombinant EGF.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human EGF as a Reference Standard.

Package:
Ultra-pure 98%: 10, 20, 50, 100 g freeze-dried powder
High-pure 95%: 100g-1g freeze-dried powder
Customer's package available

Duration:
Stable for at least three months at 2° to 8°C (lyophilized) or ≥three months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze-thaws

Recombinant Human Epidermal Growth Factor(rhEGF)Specification

Appearance	It is white. Add into 1ml dilute water, it can solve quickly.	Standard Packing	1mg/bag or per customer request
Activity unit	3.5×10⁴ - 1×10⁵ IU	Inventory	Normally we have Recombinant Human Epidermal Growth Factor(rhEGF)in stock
pH value	7.2±0.2
Water	< 3%	Recombinant Human Epidermal Growth Factor(rhEGF) physical parameters
Sterility test	No bacterial growth	Solubility	It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Abnormal toxicity exp	The tested animals have no abnormal reaction. They keep healthy, and have weight increase	Amino acid sequence	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.
Purity	> 98%	Biological Activity	The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg.
Identification test	Positive	Biological Activity
		Protein content	rhEGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of rhEGF as a Reference Standard.




Certificate of Analysis	Recombinant Human Epidermal Growth Factor(rhEGF)COA
Literature	Recombinant Human Epidermal Growth Factor(rhEGF)literature
MSDS	Recombinant Human Epidermal Growth Factor(rhEGF)MSDS

References:
1.	"Recombinant Human Epidermal Growth Factor Precursor Is a Glycosylated Membrane Protein with Biological Activity" Writen by BARBARA MROCZKOWSKI,' MARTHA REICH,' KATHERINE CHEN,' GRAEME I. BELL,2 AND STANLEY COHEN' Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennlessee 37232,1 and Howvard Huighes Medical Inistitiute anid Departmenits of Biochemistry, Moleciular Biology, amid Medicine, The University of Chicago, Chicago, Illinois 606372.
2.	"A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixes" Exp Cell Res 2005 Jul 1;307(1):142-52
3.	"Activation of the epidermal growth factor receptor in optic nerve astrocytes leads to early and transient induction of cyclooxygenase-2." Invest Ophthalmol Vis Sci 2005 Jun;46(6):2035-41
4.	"Influence of epidermal growth factor and insulin-like growth factor 1 on nuclear maturation and fertilization of buffalo cumulus oocyte complexes in serum free media and their subsequent development in vitro." Anim Reprod Sci 2005 Jul;87(3-4):229-39
5.	"Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor." Mol Cell Biol 2005 Jun;25(11):4466-75
6..	"Epidermal growth factor receptor-related protein inhibits cell growth and induces apoptosis of BxPC3 pancreatic cancer cells." Cancer Res 2005 May 1;65(9):3877-82


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